Coding
DnaJ/GrpE

Part:BBa_K205003

Designed by: Richard Schlegel   Group: iGEM09_UQ-Australia   (2009-09-28)


DnaJ and GrpE are chaperone proteins that assists with polypeptide folding

In conjuction with DnaK and GrpE, DnaJ assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaK, GrpE, GroEL and GroES forms the heat shock system in bacteria.

Note that GrpE is used to catalyse the dephosphorylation of ATP to ADP, and changes the protein conformation of the DnaK/DnaJ complex to faciliate efficient polypeptide folding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1605
    Illegal AgeI site found at 1697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1378
    Illegal SapI.rc site found at 759


[edit]
Categories
Parameters
None